β-Mannanase

EC Molecular formula: EC 3.2.1.78

Description

β-Mannanase, a member of the hemicellulase family, is produced through submerged fermentation of genetically engineered Bacillus subtilis strains, followed by multi-stage downstream purification. This enzyme effectively degrades non-starch polysaccharides (NSP) in plant-based feeds, reduces chyme viscosity, enhances nutrient digestibility, and hydrolyzes β-mannan into functional mannan oligosaccharides (MOS), thereby significantly improving animal immunity and production performance.

Mechanism and Function

 Substrate Specificity & Catalytic Action

    Target Substrates: β-Mannan, galactomannan, and glucomannan.

    Catalytic Mechanism: Random hydrolysis of β-1,4-glycosidic bonds (EC 3.2.1.78), generating MOS and trace free mannose.

    Nutritional Enhancement

    Cell Wall Disruption: Breaks down the plant cell wall matrix (cellulose-hemicellulose-lignin network), releasing encapsulated nutrients (starch, proteins, lipids) for improved accessibility to digestive enzymes.

    Viscosity Reduction: Degrades soluble NSP, lowering digesta viscosity and mitigating nutrient absorption barriers.

    Metabolic & Health Benefits

    Gut Microbiota Modulation: MOS acts as a prebiotic, selectively promoting beneficial bacteria (e.g., Bifidobacterium, Lactobacillus) in the hindgut while inhibiting pathogen colonization.

    Endogenous Enzyme Activation: Upregulates amylase, trypsin, and lipase activities, enhancing carbohydrate, protein, and lipid metabolism efficiency.

    Endocrine Regulation: Indirectly stimulates insulin and insulin-like growth factor-1 (IGF-1) secretion, improving glucose uptake and energy utilization.

    Mycotoxin Mitigation

    MOS binds mycotoxins (e.g., aflatoxin, zearalenone) via hydrogen bonding and hydrophobic interactions, reducing their bioavailability and toxicity.