Pectinase

EC Molecular formula: EC 3.2.1.15

Product Overview：Produced via microbial fermentation of Aspergillus niger (italicized, species lowercase) followed by extraction and purification.  

Applications: Widely used in juice clarification, wine processing, feed treatment, hemp fiber degumming, and wood preservation.  

Unit Definition:  

One pectinase activity unit (U) is defined as the amount of enzyme required to liberate 1 μmol of galacturonic acid per hour from pectin under standardized conditions (pH 3.5, 50°C).  

Mechanism of Action:  

Pectinase (EC 3.2.1.15) is a multi-enzyme complex comprising:  

- Polymethylgalacturonase (PMG): Cleaves methyl-esterified α-1,4-galacturonic acid bonds.  

- Polygalacturonase (PG): Hydrolyzes α-1,4-glycosidic bonds in de-esterified pectin.  

- Pectin lyase (PGL): Breaks esterified pectin via β-elimination.  

- Pectin esterase (PE): Removes methoxyl groups from pectin.  

This synergistic action degrades pectin polysaccharides into oligogalacturonides and galacturonic acid, reducing viscosity, enhancing filtration rates, improving juice yield and clarity, and minimizing post-processing sedimentation.